Journal: Journal of Dental Sciences

Article Title: Induction of retinal progenitors and neurons from dental follicle stem cell under defined conditions

doi: 10.1016/j.jds.2025.03.008

Figure Lengend Snippet: Figure 4 Immunofluorescence staining and Western blot analysis of neurogenic differentiation of dental follicle stem cells (DFSCs). Fluorescence microscopy observations for control cells (A, F, K, and P) and neurogenic induction of DFSCs on days 1, 3, 5, and 7 were presented. Staining with Nestin (red) (AeE), TUBB3 (green) (FeJ), GFAP (red) (KeO), NeuN (green) (PeT), and DAPI (blue) nuclear staining. Scale bars, 100 mm. Western blot analysis was performed with TUBB3, Nestin, NeuN, CNPase, and GFAP (U), with quantitative analysis on day 3 showing significant differences in the differentiated group compared to the control group (V). Results represent mean SEM (n Z 3). ***P < 0.001,****P < 0.0001. Abbreviations: TUBB3- tubulin beta 3, GFAP- glial fibrillary acidic protein, NeuN- neuronal nuclei, DAPI- 40,6-diamidino-2-phenylindole, CNPase- 20,30-cyclic nucleotide 30-phosphodiesterase.

Article Snippet: Membranes were incubated with primary antibody solutions of Nestin, Ceh-10 homeodomain-containing homolog (CHX10) (Abcam), TUBB3, NeuN, GFAP (Cell Signaling Technology), RAX, MITF, PAX6 (Abcepta), and 20,30-cyclic nucleotide 30-phosphodiesterase (CNPase) (Proteintech, Rosemont, IL, USA) at 4 C overnight.

Techniques: Staining, Western Blot, Fluorescence, Microscopy, Control

Journal: Journal of Dental Sciences

Article Title: Induction of retinal progenitors and neurons from dental follicle stem cell under defined conditions

doi: 10.1016/j.jds.2025.03.008

Figure Lengend Snippet: Figure 6 Immunofluorescence staining and Western blot analysis of retinal progenitor differentiation of dental follicle stem cells (DFSCs) on day 21. Fluorescence microscopy observations were presented for control cells (AeE) and retinal progenitor induction of DFSCs (FeJ). Staining with MITF (green) (A and F), RAX (green) (B and G), PAX6 (green) (C and H), TUBB3 (green) (D and I), GFAP (red) (E and J), and DAPI (blue) nuclear staining. Scale bars, 100 mm. Western blot analysis was performed with TUBB3, RAX, PAX 6, MITF, CHX10, and GFAP (K), with quantitative analysis showing significant differences compared to the control group (L). Results represent mean SEM (n Z 3). ***P < 0.001,****P < 0.0001. Abbreviations: MITF- microphthalmia-associated tran- scription factor, PAX6-paired box 6, TUBB3- tubulin beta 3, GFAP- glial fibrillary acidic protein, DAPI- 40,6-diamidino-2- phenylindole, CHX10- Ceh-10 homeodomain-containing homolog.

Article Snippet: Membranes were incubated with primary antibody solutions of Nestin, Ceh-10 homeodomain-containing homolog (CHX10) (Abcam), TUBB3, NeuN, GFAP (Cell Signaling Technology), RAX, MITF, PAX6 (Abcepta), and 20,30-cyclic nucleotide 30-phosphodiesterase (CNPase) (Proteintech, Rosemont, IL, USA) at 4 C overnight.

Techniques: Staining, Western Blot, Fluorescence, Microscopy, Control

Journal: Journal of Integrative Agriculture

Article Title: Critical role of cytochrome c1 and its cleavage in porcine reproductive and respiratory syndrome virus nonstructural protein 4-induced cell apoptosis via interaction with nsp4

doi: 10.1016/s2095-3119(17)61670-8

Figure Lengend Snippet: Fig. 1 Porcine reproductive and respiratory syndrome virus (PRRSV) nonstructural protein 4 (nsp4) interacts with cytochrome c1 (cyto.c1). A, yeast two hybrid assay. The plasmids pGBKT7-nsp4 and pGADT7-cyto.c1 were co-transformed into the yeast strain Y2HGold and selected on QDO/X/ABA plates. The top and second panels are the respective positive and negative controls. B, HEK 293T cells were transfected to express Flag-cyto.c1, or HA-nsp4 or together. At 24 h post-transfection, the cells were either subjected to direct Western blot analysis or lysed and immunoprecipitated with anti-HA antibodies. The proteins bound to sepharose beads were separated by SDS-PAGE, transferred to PVDF membranes, and probed with the antibodies to Flag and HA. C, the same as B except that nsp4 truncation mutants were used. D, MARC-145 cells were transduced with letiviruses that were expressing GFP or nsp4-GFP in the presence of 8 µg mL–1 polybrene, respectively. The cells were harvested 48 h post-transduction and cell lysates were immunoprecipitated using anti-GFP beads. The proteins bound to sepharose beads were subjected to Western blot analysis using an anti-cytochrome c1 polyclonal antibody or anti-GFP mAb. E, PRRSV nsp4 interacts with cyto.c1 in mammalian cells. Vero cells grown on coverslips in six-well plates were transfected when 60–70% to express the indicated proteins, either alone or pairwise. At 18–24 h post-transfection, the cells were fixed, permeablized, and stained with antibodies to HA and Flag, and examined by confocal microscopy. For double transfections, the merged images are shown at the right.

Article Snippet: Mouse anti-green fluorescent protein (GFP) mAb (66002-1-Ig) and rabbit anti-cytochrome c1 polyclonal antibodies (10242-1-AP) were purchased from Proteintech (Chicago, IL, USA).

Techniques: Virus, Y2H Assay, Transformation Assay, Transfection, Western Blot, Immunoprecipitation, SDS Page, Transduction, Expressing, Staining, Confocal Microscopy